E. coli Growing
Name: Sonia T.
What are the best growing
conditions for culturing
Escherichia coli bacteria using agar in a petri
dish? I'm thinking about perhaps
conducting an experiment where I'd test the effects
of various saccharides (mono-, di- and poly-) to
see how they will effect the growth of E. Coli.
Also, could you suggest any websites that will
provide me with information about Escherichia coli
without gettingcarried away with info about the
disease outbreaks, and rather on how they
obtain energy for biological processes?
I'd also like tofind out more information of the
uses of saccharides in cells.
E. coli is normally cultured on agar plates with Luria
broth (LB) or Heart Infusion (HI) medium. These
culture media are bought as powders with the agar
already mixed in. They are rich in components so
bacteria grow fast on them.
If you want to test growth on different sugars, these
media can not be used since they already contain
sugars. Your additional sugars would have no effect.
You'd have to make agar plates of minimal medium where
you can add the sugars of choice, however producing
minimal medium plates is quite complex.
There are media avaialable with specific sugar
components in them, a bacteriology lab would have
them. If you have a lab like that in your neighborhood
maybe you can even get some ready-made plates from
Here is a good on-line lecture on glucose metabolism in
A more general tutorial on metabolism of sugars can be
Compare autotrophic bacteria with heterotrophs at
I hope this will get you started.
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Update: June 2012