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Name: Adil
Status: Educator
Age: 30s
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Question:
Please give details concerning a quick method for authenticating Agrobacterium tumefaciens.



Replies:
Many bacterial species are identified nowadays by PCR, which is fast and reliable. See for instance for an A. tumefaciens- specific PCR J. Appl. Microbiol. (1999) 86:591-602. If this is not available to you I am afraid a positive identification depends on the tedious bacteriological tests that can be found inBergey's Manual or any of the other systematic bacteriology textbooks. Knowing that this is of little help to you directly, maybe this interesting site on A. tumefaciens and Crown gall makes up for it:

http://helios.bto.ed.ac.uk/bto/microbes/crown.htm

Trudy Wassenaar



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