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Name: Amy
Status: Other
Age: 20s
Location: N/A
Country: N/A
Date: October 2002


Question:
I want to conduct a hand washing experiment in which I vary scrub time, soap amt, & temp of H20. What type of nutrient agar plate should I use? I don't know which type to order...there are so many. Also, what conditions do the agar plates need to be in pre and during the experiment? thanks, Amy



Replies:
Plain nutrient agar should be fine. You will want to have the agar in a liquid state (molten, temp. 45-50 degrees C-any cooler and it will solidify). The agar can be melted by boiling, and then held in a water bath at 45 degrees until use. Then pour it into a sterile plastic petri dish and put your fingertips into the molten agar. Let the agar solidify and then incubate it upside down in an incubator at 37 degrees C for 24 hrs. or at room temperature for 48 hrs.

Good luck

vanhoeck



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