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Name: Kristian B.
Status: Student
Age: 17
Location: N/A
Country: N/A
Date: March 2004


Question:
Where in E-coli does respiration take place. Will a detergent effecting the cell membrane effect oxidative phosphorilation?



Replies:
In eukaryotes oxidative phosphorylation takes place on the inner membrane of the mitochondria. In bacteria since there are no organelles, this takes place at the cell membrane. That sounds like an interesting problem to investigate.

vanhoeck


Dear Kristian:

Additionally, it has been fairly well-documented that mitochondria originated as endosymbiotic bacteria, whose main contribution was to carry out these respiratory functions for their eucaryotic cellular hosts, as described in the following section entitled, "Both Mitochondria and Chloroplasts Probably Evolved from Endosymbiotic Bacteria" in the online textbook, Molecular Biology of the Cell @ N.C.B.I.:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=mitochondria+AND+evolution+AND+cell%5Bbook%5D+AND+7633%5Buid%5D&rid=cell.section.3592#3621

Most detergents will disrupt membranes to some extent, so this does sound like an interesting experiment to try.

Best of luck with your investigation,

Jeff Buzby, Ph.D.
CHOC Research Institute



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