Insertion of DNA
Name: Asam R.
Date: March 2004
In bacterial transformation, the plasmid serves to hold
the piece of foreign DNA to be inserted. Why can't we simply insert the
single stranded DNA directly into the bacteria?
Bacteria don't have a nucleus to store their DNA. In the cytoplasm it is subject to enzymes
which attempt to break it down from the ends called exonucleases. By forming their DNA into
a circle the ends are protected.
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Update: June 2012