RNAse and Transformation
Country: United States
Date: December 2005
If you are testing the DNA transforming principle
and you add RNAse to the DNA does it effect the transforming
The short answer is no, but that will not get you the grade. The key
to the answer is what is the target of RNase enzyme and is this similar
or distinct to the DNA transforming principle.
Jim Tokuhisa, Ph.D.
RNAse does not inactivate the transforming principle, however, DNAse
does. This statement assumes that the RNAse is pure and is not
contaminated with DNAse.
Ron Baker, Ph.D.
No, it doesn't, as long as the RNAse is sufficiently pure so as to not
contain any DNAse. In practice, before really good pure RNAse was
available, we would prepare a batch of RNAse by boiling the heck out
of it. The boiling would destroy any contaminating DNAse enzyme
activity, but not the RNAse activity, since RNAse is a remarkably
Paul Mahoney, PhD
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Update: June 2012