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Name: Larissa
Status: Student
Grade:  6-8
Location: N/A
Country: United States
Date: February 2006
Question:
Why must the temperature of the agar be no lower than 45 C and no higher than 50 C when being poured into the plates?


Replies:
Below 45 the agar begins to solidfy and above 50 the bacteria can be killed. If you are adding anything to the media, such as antibiotics, etc., some of these can be inactivated at temps higher than 50.

vanhoeck

I know of no reason to not pour at higher than 50 C, pouring at lower than certain temperatures can cause lumpiness

PF


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