Date: January 2007
When we say intron, does it represent sequence of
RNA that is cut off during RNA splicing or sequence of DNA that
after transcription are cut off during RNA splicing?
The word intron refers to a DNA sequence whose transcript will be
spliced out during mRNA processing.
Either. It is the sequence of DNA/RNA that is not found in the final
processed RNA. You can search for intronic sequences in DNA for example.
If you know the final translation of the protein you can compare the number
of bases in DNA to the number of amino acids in the protein and if there
were no introns, the DNA should be exactly 3 times the length of the protein
chain. But it isn't. So the DNA contains extra letters that don't make it
into the protein and represent the introns in the DNA.
Intron refers to the region of a gene (DNA) that does not code for the
amino acid sequence of the corresponding protein. It is the template for the
synthesis of the region of mRNA that will spliced out during mRNA
Ron Baker, Ph.D.
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Update: June 2012