Name: Ryan
Status: Other
Grade: Other
Location: MI
Country: United States
Date: Winter 2009 - 2010
Question:
If you have two containers of multiple strands unknown
cDNA, is there a way to weed-out all of the cDNA that the containers
have in common and leave only the unique strands intact?
Replies:
Some context would help on this -- what are you trying to achieve, and
what materials are you working with? Is this a 'hypothetical' question
or are you trying to actually do this?
I imagine there are lots of possible routes to achieving this: a
combination of synthesis/probes, hybridization and/or chromatography,
and PCR all might probably get the job done. This would take some work
to develop and optimize a protocol, and would depend on the nature of
the DNA in question.
Again, some context here (not only your project, but also your grade
level and what lab facilities to which you have access) would help.
If you're asking for some one-step "magic bullet" that works
generally, I don't have that.
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