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Name: Aimee
Status: student
Grade: other
Location: Outside U.S.
Country: New Zealand
Date: Spring 2011


Question:
When genes are cloned into plasmid vectors,why is it crucial to know which direction the inserted gene is facing within the plasmid?



Replies:
Hi, It is important that, the gene is inserted in right/desired direction. Only then, it can be transcribed properly. Just a simple example: TTC codes for the phenylalanine, whereas, CTT codes for leucine. When it is not inserted in right direction, you would not get your desired protien of interest. Regards.

N.Arun Prasanna. ME (PhD)., Doctoral Student, IIT-Bombay, INDIA



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