Department of Energy Argonne National Laboratory Office of Science NEWTON's Homepage NEWTON's Homepage
NEWTON, Ask A Scientist!
NEWTON Home Page NEWTON Teachers Visit Our Archives Ask A Question How To Ask A Question Question of the Week Our Expert Scientists Volunteer at NEWTON! Frequently Asked Questions Referencing NEWTON About NEWTON About Ask A Scientist Education At Argonne Plasmid Manipulation

Name: Aimee
Status: student
Grade: other
Location: Outside U.S.
Country: New Zealand
Date: Spring 2011

When genes are cloned into plasmid vectors,why is it crucial to know which direction the inserted gene is facing within the plasmid?

Hi, It is important that, the gene is inserted in right/desired direction. Only then, it can be transcribed properly. Just a simple example: TTC codes for the phenylalanine, whereas, CTT codes for leucine. When it is not inserted in right direction, you would not get your desired protien of interest. Regards.

N.Arun Prasanna. ME (PhD)., Doctoral Student, IIT-Bombay, INDIA

Click here to return to the Molecular Biology Archives

NEWTON is an electronic community for Science, Math, and Computer Science K-12 Educators, sponsored and operated by Argonne National Laboratory's Educational Programs, Andrew Skipor, Ph.D., Head of Educational Programs.

For assistance with NEWTON contact a System Operator (, or at Argonne's Educational Programs

Educational Programs
Building 360
9700 S. Cass Ave.
Argonne, Illinois
60439-4845, USA
Update: June 2012
Weclome To Newton

Argonne National Laboratory