Location: Outside U.S.
Country: New Zealand
Date: Spring 2011
When genes are cloned into plasmid vectors,why is it
crucial to know which direction the inserted gene is facing within
It is important that, the gene is inserted in right/desired direction. Only then, it can be transcribed properly. Just a simple example: TTC codes for the phenylalanine, whereas, CTT codes for leucine. When it is not inserted in right direction, you would not get your desired protien of interest.
N.Arun Prasanna. ME (PhD).,
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Update: June 2012