Department of Energy Argonne National Laboratory Office of Science NEWTON's Homepage NEWTON's Homepage
NEWTON, Ask A Scientist!
NEWTON Home Page NEWTON Teachers Visit Our Archives Ask A Question How To Ask A Question Question of the Week Our Expert Scientists Volunteer at NEWTON! Frequently Asked Questions Referencing NEWTON About NEWTON About Ask A Scientist Education At Argonne Gene Mapping

Name: Ma'ani
Status: educator
Grade: N/A
Location: Outside U.S.
Country: Somalia
Date: Winter 2013-14

Question: How do scientist actually create a gene mapping of any organism?

This is a very difficult question only because there would be so many parts to it. I will try to summarize it.

First, is extracted total DNA and RNA is extracted from the organism by any number of ways. Then the DNA is subjected to a number of procedures where the DNA is "cut" with enzymnes into smaller parts. These parts are then subjected to amplification by special techniques and sequenced by Sanger sequencing or now by NextGen sequencing, a procedure that "looks at" all the pieces of DNA. Special math modeling software combine all the data from these pieces of DNA and come up with a specific pattern of the bases on the DNA molecule. Special computers and statistical software reconstruct the data to present a "picture" or profile of the DNA of the organism. RNA which is single stranded is a bit more complicated but similar. If we consider DNA as the book of life, then RNA is a page from it.

====================== Stephen R. Dunn Ass't Professor of Medicine (ret.) Dept. Medicine/ Div. Nephrology & Cancer Genomics Kimmel Cancer Center Thomas Jefferson University

Click here to return to the Molecular Biology Archives

NEWTON is an electronic community for Science, Math, and Computer Science K-12 Educators, sponsored and operated by Argonne National Laboratory's Educational Programs, Andrew Skipor, Ph.D., Head of Educational Programs.

For assistance with NEWTON contact a System Operator (, or at Argonne's Educational Programs

Educational Programs
Building 223
9700 S. Cass Ave.
Argonne, Illinois
60439-4845, USA
Update: November 2011
Weclome To Newton

Argonne National Laboratory